FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001983724

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001983724
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7852390
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC394960.5029806211866706TruSeq Adapter, Index 4 (100% over 50bp)
GCCTACAGCACCCGGTATTCCCAGGCGGTCTCCCATCCAAGTACTAACCA203640.25933505595111805No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT140340.1787226564141618No Hit
CCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATC127750.16268932134038172No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA97430.12407687341051576No Hit
CTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATT96410.12277790583503877No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG94940.12090586432920424No Hit
CCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCA88810.11309932390011193No Hit
CCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTG88080.11216967063530978No Hit
GTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTC83560.10641346137927434No Hit
CCCCGGTTCGCCTCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGA79690.10148502557819977No Hit
CCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGA78570.10005870824042107No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)