FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001983881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001983881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7346852
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT971471.32229422887517No Hit
CCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTC810011.1025266331756787No Hit
CCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGC777381.0581130530463934No Hit
CACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCG750261.021199283720429No Hit
CCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGG472880.6436498244418154No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN449920.6123983442159988No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG435360.5925803323654811No Hit
GGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCA341850.465301329059031No Hit
CGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG287840.39178684966023547No Hit
ACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGT256270.34881606434973783No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC226610.3084450319674331No Hit
GCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGC208100.2832505677261499No Hit
AGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATC179480.24429510761888223No Hit
CCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTC169070.23012577359663705No Hit
CTGCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATT156560.2130980724805672No Hit
CCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTA153420.20882413311170556No Hit
GGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCC152390.2074221721085439No Hit
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA148100.20158293647401637No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA145560.19812567341767603No Hit
CTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCC144780.19706399421139828No Hit
CTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAATGTG140990.19190532217063852No Hit
CCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCA135100.1838882830360541No Hit
GTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTT122820.16717364117311742No Hit
GGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGG121170.16492778131368374No Hit
GGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCAT120960.16464194460430126No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA101220.13777329392234933No Hit
CACTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAATG96100.13080432272216727No Hit
TACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGG94660.12884429957211604No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC92770.12627176918767385No Hit
ACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGG91260.12421646713449515No Hit
GCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTAGT89640.12201144109068755No Hit
CATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAG81870.11143548284353626No Hit
GCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTAA80070.10898545390597225No Hit
CTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG77380.10532402177150159No Hit
ATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCA77070.10490207234336557No Hit
TGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCGG76660.10434401019647599No Hit
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT74870.10190759253078735No Hit
GTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCA74350.10119980639326884No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)