FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001984016

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001984016
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6733375
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATT810041.2030222585256278No Hit
GCCTACAGCACCCGGTATTCCCAGGCGGTCTCCCATCCAAGTACTAACCA709201.0532608090296471No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA462510.6868917890359589No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA445420.6615107579779828No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG357550.5310115655224905No Hit
GTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAAC315370.4683683888094752No Hit
CCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAAT271050.4025470139417454No Hit
CTACAGCACCCGGTATTCCCAGGCGGTCTCCCATCCAAGTACTAACCAGG231710.3441216329106875No Hit
GTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGC181140.2690181372640021No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT179070.265943898862012No Hit
GGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTA174290.2588449328902668No Hit
GGCCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAA173290.2573597935656339No Hit
CTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGA158910.2360034900774129No Hit
CCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTC155440.23085005662093674No Hit
CGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATT141680.21041453951398817No Hit
GCCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAAG138790.2061224868657991No Hit
GTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTC136910.20333042493548928No Hit
CAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGG136090.2021126106892903No Hit
CCTACAGCACCCGGTATTCCCAGGCGGTCTCCCATCCAAGTACTAACCAG132110.19620175617725139No Hit
GTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGG128480.190810700428834No Hit
CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCG121120.1798800749995359No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA120570.1790632483709878No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT118910.1765979170920972No Hit
GGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAA116560.1731078396792099No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG114700.17034548053539272No Hit
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCT113610.16872667867154287No Hit
GTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGC111220.16517719568567027No Hit
CCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAAGG109980.16333562292312548No Hit
GCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCG108510.16115246811591513No Hit
CCGGTATTCCCAGGCGGTCTCCCATCCAAGTACTAACCAGGCCCGACCCT107550.15972673436426754No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC106330.15791486438821542No Hit
CTCGATCAGAAGGACTTGGGCCCCCCACGAGCGGCGCCGGGGAGCGGGTC101840.15124658882061373No Hit
GTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAA98160.1457812761059647No Hit
GGCTAGTTGATTCGGCAGGTGAGTTGTTACACACTCCTTAGCGGATTCCG95470.1417862513227022No Hit
TGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTT93660.13909814914511667No Hit
CCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACTAGAGTC93590.1389941893923924No Hit
CCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATC92160.13687044015816735No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA91340.13565262591196836No Hit
CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTT89700.1332169974195704No Hit
GGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATT86940.12911801288358363No Hit
GGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTC86480.12843484879425252No Hit
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAAC85770.12738039987376318No Hit
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTT84670.12574674661666696No Hit
GTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGC84440.1254051645720014No Hit
GCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAA83770.12441012122449738No Hit
CACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCA82510.12253884567545992No Hit
CTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT81650.12126162585627565No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCA78030.11588542150110458No Hit
GCGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGAT77000.1143557279967327No Hit
CCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTG76920.11423691685076207No Hit
CGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAA76720.11393988898583547No Hit
CACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAA74530.11068743386488944No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC73860.10969239051738541No Hit
CTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACTAGAG73090.10854883323741808No Hit
CGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTC72670.10792507472107227No Hit
CCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATA70640.10491024189206749No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG70590.10483598492583585No Hit
CTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTG70410.10456865984740193No Hit
GCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCG69240.10283104683758144No Hit
CCTCGATCAGAAGGACTTGGGCCCCCCACGAGCGGCGCCGGGGAGCGGGT67890.10082610874932706No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)