FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001984020

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001984020
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6851655
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATT815161.1897271535125455No Hit
GCCTACAGCACCCGGTATTCCCAGGCGGTCTCCCATCCAAGTACTAACCA710441.036888167895202No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA469470.6851921178167902No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA444390.6485878229420483No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG355700.5191446446150602No Hit
GTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAAC318090.46425279731685265No Hit
CCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAAT277410.40488028074968746No Hit
CTACAGCACCCGGTATTCCCAGGCGGTCTCCCATCCAAGTACTAACCAGG234450.34218010101209123No Hit
GTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGC182090.26576060820341946No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT178210.2600977428081244No Hit
GGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTA176190.25714954999923373No Hit
GGCCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAA173900.2538072918148973No Hit
CTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGA158130.23079095488608226No Hit
CCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTC156400.22826601748044814No Hit
CGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATT141740.20686972709513246No Hit
GTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTC138750.20250581793741806No Hit
GCCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAAG137050.20002466557350013No Hit
CAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGG135660.19799595864064962No Hit
GTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGG129600.1891513802139775No Hit
CCTACAGCACCCGGTATTCCCAGGCGGTCTCCCATCCAAGTACTAACCAG129470.1889616450332073No Hit
CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCG122210.17836566493788727No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA121260.1769791386168743No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT118620.17312605494584885No Hit
GGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAA116330.16978379676151237No Hit
GTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGC113280.1653323175203655No Hit
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCT113030.16496744217273052No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG111320.16247169479490722No Hit
CCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAAGG110100.16069110309844847No Hit
GCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCG107730.1572320848028688No Hit
CCGGTATTCCCAGGCGGTCTCCCATCCAAGTACTAACCAGGCCCGACCCT107310.15661909421884201No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC106170.15495526263362647No Hit
CTCGATCAGAAGGACTTGGGCCCCCCACGAGCGGCGCCGGGGAGCGGGTC103970.1517443595744386No Hit
GTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAA97890.1428705911199557No Hit
TGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTT96750.14120675953474016No Hit
GGCTAGTTGATTCGGCAGGTGAGTTGTTACACACTCCTTAGCGGATTCCG95570.139484547893903No Hit
CCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACTAGAGTC93460.13640499995986372No Hit
CCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATC93290.13615688472347193No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA90620.13226001601073026No Hit
CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTT89880.1311799849817307No Hit
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAAC87150.12719554618555665No Hit
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTT87120.1271517611438404No Hit
GGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTC86900.12683067083792166No Hit
GGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATT86820.12671391072667845No Hit
GCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAA85570.12488953398850351No Hit
GTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGC82800.12084671513670786No Hit
CACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCA82550.12048183978907287No Hit
CTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT82020.11970830405208668No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCA79650.11624928575650699No Hit
GCGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGAT76400.11150590623725216No Hit
CCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTG75640.11039668518044181No Hit
CGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAA74440.10864528351179387No Hit
CGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTC74250.10836797824759128No Hit
CTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACTAGAG74210.10830959819196968No Hit
CACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAA73910.10787174777480768No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC72910.10641224638426774No Hit
CCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATA71630.1045440846043766No Hit
CCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGG70100.10231104747685049No Hit
CTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTG69960.1021067172821749No Hit
CCTCGATCAGAAGGACTTGGGCCCCCCACGAGCGGCGCCGGGGAGCGGGT69630.1016250818232967No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG69510.10144994165643191No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG69350.1012164214339455No Hit
GCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCG68670.10022396048837835No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)