FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001984380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001984380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9157575
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATC518140.5658048118634027No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT454260.49604835341233894TruSeq Adapter, Index 15 (97% over 40bp)
CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT377630.4123689950669255No Hit
CCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCA274320.2995552861974922No Hit
GTCGGTTCGGTCCTCCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGG259270.2831208043614167No Hit
CCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCG240060.262143635187263No Hit
GTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTC234630.25621411782049286No Hit
CCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGA221870.24228029800465734No Hit
CCCCGGTTCGCCTCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGA205720.22464462480514766No Hit
CCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTG202050.22063701361987206No Hit
CTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTT182050.198797170648343No Hit
GTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCA145240.15860093965924385No Hit
GTTGATTTCTTTTCCTCGGGGTACTTAGATGTTTCAGTTCCCCCGGTTCG143560.15676639284963542No Hit
CCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCG136540.14910060796662872No Hit
CACCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATT134920.14733158068593485No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC128460.14027731140613098No Hit
CCCCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGT126820.1384864442824656No Hit
CTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCG119350.1303292629325995No Hit
CCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGG115670.12631073182583819No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGC114960.1255354174003489No Hit
GTCCGCTTGCTCTCGCGAGGTCGCTTCTCTTTGTATGCGCCATTGTAGCA114350.12486930218971726No Hit
GCTTTCTTTAAATGATGGCTGCTTCTAAGCCAACATCCTGGCTGTCTGGG107800.1177167536165415No Hit
CCTTGGTCTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATG103050.11252979091080335No Hit
GTACGATTTGATGTTACCTGATGCTTAGAGGCTTTTCCTGGAAGCAGGGC97860.10686235165969157No Hit
CCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTA97220.10616347668460265No Hit
GGCATTTGTTGCTTCAGCACCGTAGTGCCTCGTCATCACGCCTCAGCCTT96390.10525712320128419No Hit
CCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTT94490.10318233811898891No Hit
CTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTA94220.10288750023887329No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)