FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001984383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001984383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9146561
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA487310.5327794785384364No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG460990.504003635902062Illumina Single End PCR Primer 1 (100% over 50bp)
GGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACA313460.3427080407598003No Hit
GGGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAAC289280.31627187529826783No Hit
CTGCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATT247140.2701999144815193No Hit
CATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAAC237340.25948550498925227No Hit
CTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGT168730.18447370547247213No Hit
GTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAA165670.18112818577386627No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA145380.15894498489650918No Hit
CCCGGTTTAAGCGTGTAGGCTGGTTTTCCAGGCAAATCCGGAAAATCAAG144510.1579938077272977No Hit
AAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGG142360.15564319748154526No Hit
GCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAG140830.15397043763224233No Hit
GGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGAT137620.15046092186997934No Hit
CATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTGGGTAGGGGAG137200.15000173289173932No Hit
GTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGG128980.14101474860332752No Hit
CGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGA125700.13742870134469118No Hit
CGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATG125100.13677271709006258No Hit
GTCCAACGTTAATCGGGGCAGGGTGAGTCGACCCCTAAGGCGAGGCCGAA116510.12738120917796317No Hit
GGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGTATAGACCCGAAA103000.11261063037790926No Hit
CTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTG102780.11237010281787876No Hit
CGTTAATCGGGGCAGGGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGT101880.11138612643593587No Hit
CGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGT101370.11082853981950157No Hit
GCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGGCAG101360.11081760674859108No Hit
CTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGACGTCA100570.10995389414666341No Hit
CGAAGGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGTATAGACCC96830.10586492562614518No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)