FastQCFastQC Report
Sat 11 Mar 2023
EGAF00001984862

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001984862
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94283143
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTC3996990.42393474303248463No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT3283600.3482701038084825TruSeq Adapter, Index 1 (97% over 36bp)
GCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGC3178070.3370772228074747No Hit
GGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCG3149940.33409365659352275No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3065550.3251429579516669No Hit
GTGGGTTCTAGGTTAGCGCGCAGTTGGGCACCGTAACCCGGCTTCCGGTT2079500.22055904521553765No Hit
CTGCAAAGGATTCTACCCGCCGCTCGGTGGGAATTGTACTTCAAGGCGGC1797060.19060247068768166No Hit
GTTCTAGGTTAGCGCGCAGTTGGGCACCGTAACCCGGCTTCCGGTTCATC1420130.15062395618270807No Hit
GCCCTTAGCTTTTTGTTTCCTAGCTTGTCTTTTTTCTTCTGCTTCCTACT1380940.14646732767489518No Hit
CCCCATCACGATGAAATTTCAAAGATTACCCGGGCCTGTCGGCCAAGGCT1321810.14019579300617926No Hit
GCAAAGGATTCTACCCGCCGCTCGGTGGGAATTGTACTTCAAGGCGGCCC1242860.13182207979638522No Hit
GTCGGCATCGTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAG1207380.1280589468681586No Hit
CGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACT1193650.1266026950331938No Hit
GGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGC1187660.12596737467693456No Hit
CCTCGTTGAAGACCAACAATTGCAATGATCTATCCCCATCACGATGAAAT1160410.12307714434169849No Hit
CTTAGCTTTTTGTTTCCTAGCTTGTCTTTTTTCTTCTGCTTCCTACTTTT1149910.12196347760702038No Hit
GTCGTCTGCAAAGGATTCTACCCGCCGCTCGGTGGGAATTGTACTTCAAG1135940.12048177053240577No Hit
CCCATCACGATGAAATTTCAAAGATTACCCGGGCCTGTCGGCCAAGGCTA1128980.119743568582562No Hit
CCTCATTTTTTCAGTGCTATTTTATCCAATTTTTGGCTTTATATTTTTCT1121080.1189056669440899No Hit
GCTGGATAGTAGACAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGC1039180.11021906641360057No Hit
CTCATTTTTTCAGTGCTATTTTATCCAATTTTTGGCTTTATATTTTTCTA999680.10602955822124004No Hit
GCCGGGGCACGCCGTGACGGCGCGCGCTGCAGTCCACGATCGCGGCGACG978850.10382025554663574No Hit
CCTAGCTTGTCTTTTTTCTTCTGCTTCCTACTTTTCAGGTTTAAATTTAT954060.10119094141780996No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)