FastQCFastQC Report
Thu 23 Feb 2023
EGAF00001984867

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001984867
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111552859
Sequences flagged as poor quality0
Sequence length126
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8111660.7271584137525332No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT5178870.46425255671842536Illumina Single End PCR Primer 1 (96% over 32bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2728100.24455670831350007No Hit
CGCAAAACTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT1151820.10325329268342644No Hit
AAATAATTCAAGCACTGCTTATTACAATTTTACTGGGTCTCTATTTTACC1118960.10030760394944248No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)