FastQCFastQC Report
Thu 23 Feb 2023
EGAF00001984872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001984872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106603946
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT5387560.5053809171379078TruSeq Adapter, Index 13 (97% over 38bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2709980.2542101021288649No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1402600.13157111463772644No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)