FastQCFastQC Report
Thu 9 Feb 2023
EGAF00001984877

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001984877
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110725336
Sequences flagged as poor quality0
Sequence length126
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCT5193160.46901280118942246Illumina Single End PCR Primer 1 (96% over 33bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1265570.11429814040031451No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)