FastQCFastQC Report
Thu 5 Jan 2023
EGAF00001984882

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001984882
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79312234
Sequences flagged as poor quality0
Sequence length126
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT12734291.6055896244203636TruSeq Adapter, Index 3 (97% over 36bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7456800.9401828222364788No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5329410.6719530810341315No Hit
CTTAGCTTTTTGTTTCCTAGCTTGTCTTTTTTCTTCTGCTTCCTACTTTT1031230.1300215550604715No Hit
CTCATTTTTTCAGTGCTATTTTATCCAATTTTTGGCTTTATATTTTTCTA971730.12251955984495407No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)