FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001993960

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001993960
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4014339
Sequences flagged as poor quality0
Sequence length65
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGGTCAATCTCGTATGCCGTCTTCTGCTTG2025795.04638497147351TruSeq Adapter, Index 25 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGGTCAATCTCGTATGCCGTCTTCTGCTTGA88590.22068390337736798TruSeq Adapter, Index 25 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)