FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001993994

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001993994
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6141562
Sequences flagged as poor quality0
Sequence length65
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGGCATAATCTCGTATGCCGTCTTCTGCTTG1645362.679057868340334TruSeq Adapter, Index 20 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGGCATAATCTCGTATGCCGTCTTCTGCTTGA95990.1562957436560927TruSeq Adapter, Index 20 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)