FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001994006

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001994006
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7472722
Sequences flagged as poor quality0
Sequence length65
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCGTCATCTCGTATGCCGTCTTCTGCTTG2078862.7819314033092626TruSeq Adapter, Index 27 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCGTCATCTCGTATGCCGTCTTCTGCTTGA217530.291098745544127TruSeq Adapter, Index 27 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)