FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001994018

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001994018
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7470191
Sequences flagged as poor quality0
Sequence length65
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATGTACATCTCGTATGCCGTCTTCTGCTTG2257153.0215425549360115TruSeq Adapter, Index 2 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATGTACATCTCGTATGCCGTCTTCTGCTTGA160330.21462637300706236TruSeq Adapter, Index 2 (97% over 39bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)