FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001994094

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001994094
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139217
Sequences flagged as poor quality0
Sequence length35-301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG4518432.45580640295366No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA2950721.194969005222063No Hit
GCTGCGTTCTTCATCGCCTCTGACTGCCAGGGCATCCACCGTGTACGCTT1803512.95459606226251No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG79755.72846706939526No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTCAAAAGTTG79495.70979118929441No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTG12420.8921324263559766No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA12300.8835127893863537No Hit
GCTGCGTTCTTCATCGCTGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG12220.8777663647399384No Hit
GCTGCGTTCTTCATCGCTGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA6700.48126306413728215No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAATAAAATACTGGCAAACAG6590.473361730248461No Hit
GCTGCGTTCTTCATCGATGTGAGAACCAAGAGATCCGTTGTCGAAAGTTG6520.4683336086828476No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGGGATCCACCGCTAAGAGTCG4980.35771493423935297No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGCAAGTTG4440.3189265678760496No Hit
GCTGCGTTCTTCATCGATGCGCAGGTTCACCTACGGA4130.2966591723711903No Hit
GCTGCGTTCTTCATCGACGCACGAGCCGAGTGATCCACCGCTAAGAGTCG3950.28372971691675586No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGCGTCG3660.26289892757350036No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGCGATCCACCGCTAAGAGTCG3460.24853286595746207No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAACGTTG3330.23919492590703723No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGCGATCCGTTGTCGAAAGTTG2740.1968150441397243No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGCTCCGTTGTCGAAAGTTG2720.1953784379781205No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCCCCGCTAAGAGTCG2710.19466013489731857No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGGGATCCCCCGCTAAGAGTCG2630.18891371025090326No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGCTGATAGTTA2320.16664631474604394No Hit
GCTGCGTTCTTCATCGCTGCACGAGCCGAGTGATCCACCGCTAAGAGTCG2270.16305479934203437No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTCGATAGTTA2110.15156195004920375No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGCGATCCGTTGTTGATAGTTA2090.15012534388759993No Hit
GCTGCGTTCTTCATCGCTGCGAGAGCCAAGAGATCCGTTGTCAAAAGTTG1980.14222400999877888No Hit
GCTGCGTTCTTCATCGCCTCTGACTGCCAGGGCACCCACCGTGTACGCTT1780.12785794838274062No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATCGTTA1720.12354812989792913No Hit
GCTGCGCTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG1490.10702715903948513No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGCTGTCGAAAGTTG1470.10559055287788129No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGCGATCCCCCGCTAAGAGTCG1450.10415394671627745No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)