FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001994095

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001994095
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences312926
Sequences flagged as poor quality0
Sequence length35-301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAATTGCTCTTTGAGCGT11225435.872378773256294No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAAGAACGCCCGGGCTTCGG7123622.764487450707197No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG189896.068207819101001No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTCACACATCCAATCAAACCTAA156575.003419338757406No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAACCCTGCACAGCAGAA84262.6926493803646867No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCACAACCTTAATACCTTCA79452.538938918466347No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACTGTGATTTATCCACCACAC69482.2203332417248807No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGGCCA63162.0183685599790366No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGTCG54701.7480171030850744No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAATTGCTCTTTGCGCGT45781.4629656851779653No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGAATCAATCAAATACAAACCG37461.1970881294619176No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG32191.0286777065504304No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCAGAACGCCCGGGCTTCGG24290.7762218543681254No Hit
TCCGTAGGTGAACCTGCGTGCCTGTGGCTCACTCCAGGGGTGTGTTGCGT18500.5911940842243852No Hit
TCCGTAGGTGAACCTGTCAAGCATGCAGAAACAAATATATCAATTATATG15420.4927682583102715No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAATTGCTCTTTTAGCGT9090.29048401219457637No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAATTGCTCTTTGATCGT8760.2799383879894927No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATCGATTCTTATTAACCGGTC7110.2272102669640746No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGCGAATTGCTCTTTGAGCGT5350.1709669378702952No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAATGTAATAATCATTTTACAT4560.1457213526520647No Hit
TCCGTAGGTGAACCTGCGAGGACAATGGCCCATGACATTTCAGTGGCTGC4340.13869093651534228No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)