FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001994096

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001994096
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences312926
Sequences flagged as poor quality0
Sequence length35-301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTA11362136.309223266842636No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT6733821.518825537027926No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA179755.744169548072068No Hit
GCTGCGTTCTTCATCGATATGGGAGCCAAGACATCCACTGTTGAAAATTT129464.137080332091293No Hit
GCTGCGTTCTTCATCGATGCGTGAGCCGAGATATCCGTTGCCGAGAGTCG81172.593903990080722No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT64852.0723749384838586No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG59511.9017275649834144No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG57981.8528342163962086No Hit
GCTGCGTTCTTCATCGATGTGGGAGCCAAGACATCCGTTGCTGAAAGTGT39001.2463010424189744No Hit
GCTGCGTTCTTCATCGATGCGGGAGCCAAGATATCCGTTGTTGAGAGTTG35391.1309383049027566No Hit
GCTGCGTTCTTCATCGTTGTGGGAGCCAAGACATCCGTTGCTGAAAGTGT32541.0398624594952162No Hit
GCTGCGTTCTTCATCGCTGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTA27430.8765650665013454No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTGAAAGTTA21720.6940938113164135No Hit
GCTGCGTTCTTCATCGATGCCAAAGGGTTCAGGATTTCCTCCGCCCTCCC17860.5707419645539201No Hit
GCTGCGTTCTTCATCGCTGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT17450.5576398253900283No Hit
GCTGCGTTCTTCATCGATGACCTGTTCAAAGCTGTTGGTGATGGAATTGT14400.46017269258546745No Hit
GCTGCGTTCTTCATCGATTCCAGAACCAAGAGATCCGTTGTTAAAAGTTT10630.3396969251516333No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGCTAAAAGTTT10230.32691435035759253No Hit
GCTGCGTTCTTCATCGACGCACGAGCCGAGTGATCCACCGCTAAGAGTCG9180.29336009152323556No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA7810.2495797728536459No Hit
GCTGCGTTCTTCATCGATGTGGGAGCCAAGACATCCACTGTTGAAAATTT7430.23743632679930718No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGCGATCCGTTGTTGAAAGTTA6630.21187117721122567No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAACGTTT6350.2029233748553971No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTCGAAAGTTA6210.19844947367748283No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGCAAGTTA5940.18982123569150533No Hit
GCTGCGTTCTTCATCGTTATGGGAACCTAGATATCCGCCGTTGAAAATTT5860.18726472073269718No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAACGTTA5710.1824712551849319No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGCTGTTGAAAGTTA5550.1773582252673156No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGCGATCCGTTGTTAAAAGTTT5470.17480171030850744No Hit
GCTGCGTTCTTCATCGATATGGGAGCCAAGACATCCCCTGTTGAAAATTT5160.16489521484312586No Hit
GCTGCGTTCTTCATCGATTCGAGAGCCAAGAGATCCGTTGTTGAAAGTTA4220.13485616407713005No Hit
GCTGCGTTCTTCATCGCTGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA4190.13389747096757698No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGGGATCCACCGCTAAGAGTCG3780.12079533180368522No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCAAAAGTTT3730.11919750995443013No Hit
GCTGCGTTCTTCATCGACCCACGAACCAAGTGATCCCCCGCTTAGGGTAA3630.11600186625591992No Hit
GCTGCGTTCTTCATCGGTGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTA3630.11600186625591992No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTACAAGTTT3590.11472360877651584No Hit
GCTGCGTTCTTGCTGCGGTTATTGGGTCAGGGCTGGGGCAAGGATCTAAG3530.11280622255740974No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGCTCCGTTGTTAAAAGTTT3500.11184752944785668No Hit
GCTGCGTTCTTCATCGCTATGGGAGCCAAGACATCCACTGTTGAAAATTT3390.10833232137949549No Hit
GCTGCGTTCTTCATCGCTGTGGGAGCCAAGACATCCGTTGCTGAAAGTGT3380.10801275700964447No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGCTGTTAAAAGTTT3310.10577580642068732No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGCTCCGTTGTTGAAAGTTA3260.10417798457143222No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)