FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001994097

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001994097
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences277219
Sequences flagged as poor quality0
Sequence length35-301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG12844746.334125727313065No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTCAAGGGCCAGC6651023.99186202965886No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGCAGAGCAGAA198807.17122563749238No Hit
TCCGTAGGTGAACCTGCGCACCACAGCTACTTTCCCAGTCCAAGTGAGGA85243.074825318610918No Hit
TCCGTAGGTGAACCTGCCACAGCGGATTAGCGAGGCGGAACAGAGGGGAC35131.267229158174584No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG32931.1878695183230588No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAAGTATGAAGCCGACTTGATCGT27761.0013743646719742No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAAGTATGAAGCCGACTTGATTGT13670.4931119439865233No Hit
TCCGTAGGTGAACCTGCTCCAGAAACTGAAGTTTGAAATGGAGCTCCTAA8120.2929092161792662No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTCAAGGGCCAGC7910.28533397782980247No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTT6170.22256771721995966No Hit
TCCGTAGGTGAACCTGCATCGATGAAGAACGCAGC5980.2157139301418734No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTCAAGGTCCAGC5780.20849941742809835No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGCCTATACTTTTG5060.18252717165850826No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4800.17314830513060073No Hit
TCCGTAGGTGAACCTGCCGAAGGATCATTAGTGAAGATTCAAGGGCCAGC4770.17206612822353445No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGCTTTGACTATACTTTTG4140.14934041317514313No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATCCTTTTG4080.1471760593610106No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACCTTTG3480.12553252121968553No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGCGAAGATTCAAGGGCCAGC3140.11326784960626797No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)