FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001994098

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001994098
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences277219
Sequences flagged as poor quality0
Sequence length35-301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA12036243.41765896276951No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG6397023.075618915009432No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG183356.613904530353258No Hit
GCTGCGTTCTTCATCACACAGAGCCTCCTTTAGAGCACCAGGGCCTCCAC74892.70147428567306No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA47631.7181362027855234No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAATAAAATACTGGCAAACCG39281.4169302969854158No Hit
GCTGCGTTCTTCATCGATGCCGGGTGAGAGGAGAACGCGGCCTGGGGCGC33331.2022985437506086No Hit
GCTGCGTTCTTCATCGCTGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA26780.9660232523744766No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTG18460.6658995234814353No Hit
GCTGCGTTCTTCATCGCTGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG15360.5540745764179223No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG12250.4418889037187206No Hit
GCTGCGTTCTTCATCGATGTGAGAACCAAGAGATCCGTTGTCGAAAGTTG10090.3639721664099503No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGCTGATAGTTA7880.2842518009227362No Hit
GCTGCGTTCTTCATCGATGCTCCCTGCCCTGACCACCAGGCCATGCATGG7550.27234785494500735No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTCGATAGTTA7350.26513334223123236No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGCGATCCGTTGTTGATAGTTA6360.22942150429804595No Hit
GCTGCGTTCTTCATCGATGCAGGTTCACCTACGGA5930.2139103019634296No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATCGTTA5920.2135495763277409No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGCGTCG5800.20922086869947587No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGCTA5780.20849941742809835No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4830.17423048203766697No Hit
GCTGCGTTCTTCATCGGTGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA4530.16340871296700443No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGCAAGTTG4510.16268726169562692No Hit
GCTGCGTTCTTCATCGCTGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG4460.16088363351718316No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGCTGTTGATAGTTA4050.14609388245394436No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTCA4040.1457331568182556No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGCTAGTTA4020.1450117055468781No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAACGTTG3260.117596557234533No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGCTCCGTTGTCGAAAGTTG3240.11687510596315549No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGCTCCGTTGTTGATAGTTA2860.10316753180698293No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)