FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001994099

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001994099
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224529
Sequences flagged as poor quality0
Sequence length35-301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGCCTAGCAGAA8396137.39427869005785No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAATTGCTCTTTGAGCGT7222432.16689158193374No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATCGATTCTTATTAACCGGTC171887.655135862182613No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG47082.096833816567125No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACACAATCTAAAATCTCACC42931.9120024584797508No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAATTGCTCTTTGCGCGT28521.2702145379884113No Hit
TCCGTAGGTGAACCTGCCCATTGTGGAGCGGCCGGTGCCGATGAAGAACGCAGC17920.7981151655242753No Hit
TCCGTAGGTGAACCTGCGTGCCTGTGGCTCACTCCAGGGGTGTGTTGCGT10720.47744389366184325No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGCCTATCAGAA6880.3064192153352128No Hit
TCCGTAGGTGAACCTGCAGCAGTAACCACACAGGTGGACCTCTTAGGTTGGAGCTGGATGAAGAACGCAGC6140.2734613346160184No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGCCTAGCAGAC5650.25163787305871405No Hit
TCCGTAGGTGAACCTGCTCCAGAAACTGAAGTTTGAAATGGAGCTCCTAA5220.23248667210026322No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAATTGCTCTTTGATCGT5180.2307051650343608No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAATTGCTCTTTTAGCGT5090.22669677413608041No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCAAATCCAAATACCCAACC4550.20264642874639802No Hit
TCCGTAGGTGAACCTGTAGATGGATCATTAACACATCCATATCAACCTAA4110.18304985102147162No Hit
TCCGTAGGTGAACCTGCGAGGTGCTTCTAGTGTTACCTTTACAGCATCGATGAAGAACGCAGC3680.16389865006302082No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGCGAATTGCTCTTTGAGCGT3240.14430207233809442No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGCAACCTGCCTAGCAGAA3020.13450378347563122No Hit
TCCGTAGGTGAACCTGCGAGGACAATGGCCCATGACATTTCAGTGGCTGC2460.1095626845529976No Hit
TCCGTAGGTGAACCTGCGGAAGGCTCATTGTCGAAACCTGCCTAGCAGAA2460.1095626845529976No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGCCTCGCAGAA2450.10911730778652201No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAATTGCCCTTTGAGCGT2330.10377278658881481No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAACCCTGCCTAGCAGAA2320.10332740982233922No Hit
TCCGTAGGTGAACCTGCGGAAGGCTCATTAGTGAATTGCTCTTTGAGCGT2260.1006551492234856No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)