FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001994100

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001994100
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224529
Sequences flagged as poor quality0
Sequence length35-301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG8082335.99668639685742No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTA7399332.95476308182907No Hit
GCTGCGTTCTTCATCGTTATGGGAACCTAGATATCCGCCGTTGAAAATTT145576.483349589585309No Hit
GCTGCGTTCTTCATCGTTATGGGAACCAAGATATCCATCGTTGAAAATTT32801.460835794039968No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG25381.1303662333150728No Hit
GCTGCGTTCTTCATCGCTGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG21490.9571146711560644No Hit
GCTGCGTTCTTCATCGGCACCGGCCGCTCCACAATGGGCAGGTTCACCTACGGA18390.8190478735486284No Hit
GCTGCGTTCTTCATCGCTGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTA17370.7736194433681173No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTGAAAGTTA12410.5527125671962196No Hit
GCTGCGTTCTTCATCGATGCCAAAGGGTTCAGGATTTCCTCCGCCCTCCC10460.4658640977334776No Hit
GCTGCGTTCTTCATCGCTATGGGAACCTAGATATCCGCCGTTGAAAATTT9340.41598189988821044No Hit
GCTGCGTTCTTCATCGATATGGGAACCTAGATATCCGCCGTTGAAAATTT8270.36832658587532124No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGCTATCCGTTGCCGAGAGTCG7710.3433854869526876No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGCGTCG6430.2863772608438108No Hit
GCTGCGTTCTTCATCCAGCTCCAACCTAAGAGGTCCACCTGTGTGGTTACTGCTGCAGGTTCACCTACGGA5790.2578731477893724No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATCTCCGTTGCCGAGAGTCG5600.249410989226336No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGCGAGTCG5130.2284782812019828No Hit
GCTGCGTTCTTCATCGATGCTCCCTGCCCTGACCACCAGGCCATGCATGG5010.22313376000427562No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTCGAAAGTTA4470.1990834146145932No Hit
GCTGCGTTCTTCATCGGTATGGGAACCTAGATATCCGCCGTTGAAAATTT4270.1901758792850812No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGATAGTCG4050.180377590422618No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGCGATCCGTTGTTGAAAGTTA3930.1750330692249108No Hit
GCTGCGTTCTTCATCGATGCTGTAAAGGTAACACTAGAAGCACCTCGCAGGTTCACCTACGGA3600.16033563593121603No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGCAAGTTA3540.1576633753323624No Hit
GCTGCGTTCTTCATCGTTACGAGAGACGAGATATCCATCGCTGAGGTTTT3340.14875584000285041No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAACGTTA3230.14385669557161881No Hit
GCTGCGTTCTTCATCGATCCACGAGCCAAGAGATCCACCGTTAAAAGTTG2850.12693237844554603No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGCTGCCGAGAGTCG2790.1242601178466924No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGCTGTTGAAAGTTA2640.11757946634955842No Hit
GCTGCGTTCTTCATCGGTGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTA2570.11446182898422921No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGATATCCGTTGCCGAGAGTCG2440.10867193102004641No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGAGATCCGTTGCCGAGAGTCG2400.10689042395414401No Hit
GCCGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG2360.10510891688824162No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA2360.10510891688824162No Hit
GCTGCGTTCTTCATCGTTATGGGAACCTAGATATCCGCCGTTGAAACTTT2300.102436656289388No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)