FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001994101

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001994101
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences300025
Sequences flagged as poor quality0
Sequence length35-301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTCAAGGGCCAGC11257637.5222064827931No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGGCCA7242924.140988250979085No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGATAAAACTATTATCTTA3268210.893092242313141No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG173915.796516956920256No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG73122.4371302391467378No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTCAAGGGCCAGC45181.5058745104574618No Hit
TCCGTAGGTGAACCTGCCACAGCGGATTAGCGAGGCGGAACAGAGGGGAC33581.119240063328056No Hit
TCCGTAGGTGAACCTGCATCGATGAAGAACGCAGC18500.6166152820598283No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTTTTGAAGGCTACACGCCGACA16010.5336221981501542No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTTGGGCAGGCCA9870.3289725856178652No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTCAAGGTCCAGC8420.28064327972668945No Hit
TCCGTAGGTGAACCTGCCGAAGGATCATTAGTGAAGATTCAAGGGCCAGC7950.26497791850679114No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGCGAAGATTCAAGGGCCAGC6780.22598116823598033No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4730.15765352887259393No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGTCCA4420.14732105657861846No Hit
TCCGTAGGTGAACCTGCCGAAGGATCATTAGTGAAGATTTGGGCAGGCCA4380.14598783434713775No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGGCCC4150.13832180651612366No Hit
TCCGTAGGTGAACCTGCAGAAGGCTCATTAGTGAAGATTCAAGGGCCAGC3990.13298891759020082No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTCAAGGGCTAGC3790.12632280643279728No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTTGGCAGGCCA3340.11132405632863927No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGATAAAACTATTCTCTTA3010.10032497291892342No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)