FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001994102

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001994102
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences300025
Sequences flagged as poor quality0
Sequence length35-301
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG18152760.50395800349971No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG3128910.428797600199983No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA163065.434880426631114No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTG51541.7178568452628946No Hit
GCTGCGTTCTTCATCGCTGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG47781.592533955503708No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG45001.4998750104157985No Hit
GCTGCGTTCTTCATCGATGCCGGGTGAGAGGAGAACGCGGCCTGGGGCGC32111.07024414632114No Hit
GCTGCGTTCTTCATCGATGTGAGAACCAAGAGATCCGTTGTCGAAAGTTG27870.9289225897841846No Hit
GCTGCGTTCTTCATCGATGCAGGTTCACCTACGGA18210.6069494208815932No Hit
GCTGCGTTCTTCATCGATGTGAGAACCAAGAGATCCGTTGTTGAAAGTTT15130.5042913090575785No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGCAAGTTG14640.48795933672193986No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAACGTTG9820.3273060578285143No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGCGATCCGTTGTCGAAAGTTG9500.3166402799766686No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGCTCCGTTGTCGAAAGTTG9360.31197400216648613No Hit
GCTGCGTTCTTCATCGCTGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG7920.26397800183318054No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA7000.2333138905091242No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTCG5120.17065244562953086No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTGAAAGTTG4970.1656528622614782No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4740.15798683443046413No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTCG4730.15765352887259393No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGCTGTCGAAAGTTG4690.15632030664111324No Hit
GCTGCGTTCTTCATCGATGTGAGAACCAAGAGATCCGTTGTCGATAGTTG4560.15198733438880094No Hit
GCTGCGTTCTTCATCGGTGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG4100.13665527872677277No Hit
GCTGCGTTCTTCATCGATTGGAGAACCAAGAGATCCGTTGTCGAAAGTTG4070.13565536205316223No Hit
GCTGCGTTCTTCATCGCTGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA3930.13098908424297975No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTCGAAAGTTG3160.10532455628697607No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)