FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001994103

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001994103
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224333
Sequences flagged as poor quality0
Sequence length35-301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG15231667.89727770769348No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGCCCAGCAGAA4069418.139997236251464No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG12730.5674599813669857No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTT6490.28930206434184896No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGCCTATACTTTTG6390.28484440541516404No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGCTTTGACTATACTTTTG5310.23670168900696734No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATCCTTTTG4540.2023777152714937No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACCTTTG4510.20104041759348826No Hit
TCCGTAGGTGAACCTGCCACAGCGGATTAGCGAGGCGGAACAGAGGGGAC3930.17518599581871594No Hit
TCCGTAGGTGAACCTGCGGAAGGCTCATTATTGATTTGACTATACTTTTG3350.1493315740439436No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAGGGATGAAGCCCACTTGATCAT2870.12793481119585615No Hit
TCCGTAGGTGAACCTGCATCCCAGGGATTAAGCACACTTGATCTTGGTGG2860.12748904530318766No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2790.12436868405450825No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATCGATTTGACTATACTTTTG2450.10921264370377964No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGCCCAGCAGAC2420.10787534602577419No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGCCCATCAGAA2390.10653804834776873No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTC2280.10163462352841535No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)