FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001994104

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001994104
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224333
Sequences flagged as poor quality0
Sequence length35-301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA14254663.542144936322345No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG3812716.995716189771457No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA47182.103123481609928No Hit
GCTGCGTTCTTCATCGCTGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA32541.450522214743261No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGCTGATAGTTA9880.4404167019564665No Hit
GCTGCGTTCTTCATCGCTGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG9540.4252606616057379No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGCGATCCGTTGTTGATAGTTA8730.38915362429959033No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTCGATAGTTA7290.324963335755328No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATCGTTA6680.2977716163025502No Hit
GCTGCGTTCTTCATCGATTTGAGAGCCAAGAGATCCGTTGTTGATAGTTA6420.28618170309316954No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGCTA6160.27459178988378885No Hit
GCTGCGTTCTTCATCGGTGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA5510.2456170068603371No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGCTGTTGATAGTTA5440.24249664561165765No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGCTAGTTA5380.23982205025564673No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG5050.2251117757975866No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGCGTCG4890.21797952151489083No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTCA4240.18900473849143906No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGCTATCCGTTGCCGAGAGTCG3930.17518599581871594No Hit
GCTGCGTTCTTCATCGATGCCGGGTGAGAGGAGAACGCGGCCTGGGGCGC3850.17161986867736803No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGCGAGTCG3550.1582468918973134No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGCTCCGTTGTTGATAGTTA3020.13462129958588348No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATCTCCGTTGCCGAGAGTCG2930.1306094065518671No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2710.12080255691316036No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAGTAAAATACTGACAAACCA2570.11456183441580152No Hit
GCTGCGTTCTTCATCGATGCAAACATTCTCAATAAAATACTGGCAAACCG2330.10386345299175778No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGCTGATAGTCA2270.10118885763574685No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)