FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001994105

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001994105
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences389206
Sequences flagged as poor quality0
Sequence length35-301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG14428737.072141744988514No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGATAAAACTATTATCTTA8099020.80903172099094No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTCATAATTTATGCATGTTCTTC4458311.454859380379542No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTCACACATCCAATCAAACCTAA195025.010714120542849No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTGAGGGCCCTCTGGG173204.450085558804335No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTCATAATTTATGTAATGTTCTT120863.1052964240016854No Hit
TCCGTAGGTGAACCTGCCCCGATTACCGGGACAACCCGAGGAAACTCGGG76131.9560335657723673No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGAATCTATCGTGCCCACACCG75151.9308540978299409No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG16140.4146904210109813No Hit
TCCGTAGGTGAACCTGCCCCGATGACCGGGACAACCCGAGGAAACTCGGG8010.20580361042738293No Hit
TCCGTAGGTGAACCTGCCTGTGAACTCTGAAGTCACTAGTCCAGCTGGGT6850.17599934225063335No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGATAAAACTATTCTCTTA6550.16829134186009465No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTT6470.16623587508928434No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGCCTATACTTTTG5740.1474797408056402No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGCTTTGACTATACTTTTG5350.1374593402979399No Hit
TCCGTAGGTGAACCTGCGTGCTCCTGTACTGGGTGCATATACATTTAGGA4750.12204333951686254No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATCCTTTTG4340.11150907231645965No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACCTTTG4230.10868280550659548No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)