FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001994106

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001994106
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences389206
Sequences flagged as poor quality0
Sequence length35-301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA13460934.58554081900074No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG7696919.775902735312407No Hit
GCTGCGTTCTTCATCGGTATAGGAGCCTAGATATCCATCGTTGAAAATTT4998412.842556384022858No Hit
GCTGCGTTCTTCATCGATATGGGAGCCAAGACATCCACTGTTGAAAATTT162024.162834077583593No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT160954.135342209524005No Hit
GCTGCGTTCTTCATCGATGCCTTGGTGAGCCGTTACCTCACCAACTAGCT72321.858141960812526No Hit
GCTGCGTTCTTCATCGTTACGGGAGCCAAGATATCCGTTGTTGAGAGTTG62311.6009516811148852No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA50471.2967425990349584No Hit
GCTGCGTTCTTCATCGCTGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA31120.7995765738452131No Hit
GCTGCGTTCTTCATCGATATAGGAGCCTAGATATCCATCGTTGAAAATTT18030.4632508234713751No Hit
GCTGCGTTCTTCATCGCTGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG17110.43961295560705643No Hit
GCTGCGTTCTTCATCGGTATAGGAGCCTAGATATCCCTCGTTGAAAATTT15620.4013298870007143No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTGAAAGTTG11620.29855654846019847No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGCTGATAGTTA9090.2335524118333222No Hit
GCTGCGTTCTTCATCGGTATAGGAGCCTAGATATCCATCGTTGAAACTTT8850.22738601152089127No Hit
GCTGCGTTCTTCATCGATGTGGGAGCCAAGACATCCACTGTTGAAAATTT8740.22455974471102705No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG8430.2165948109741371No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTCGATAGTTA7670.1970678766514391No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGCGATCCGTTGTTGATAGTTA7350.18884600956819783No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATCGTTA6430.16520814170387918No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTCGAAAGTTG6310.1621249415476637No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAACGTTG6130.1575001413133405No Hit
GCTGCGTTCTTCATCGATACGGGAGCCAAGATATCCGTTGTTGAGAGTTG5840.1500490742691531No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGCTA5790.14876440753739664No Hit
GCTGCGTTCTTCATCGATATGGGAGCCAAGACATCCCCTGTTGAAAATTT5750.1477366741519915No Hit
GCTGCGTTCTTCATCGGTGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA5660.14542427403482988No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGCAAGTTG5280.1356608068734809No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTCA4810.12358493959497027No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGCTGTTGATAGTTA4770.12255720620956512No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGCGATCCGTTGTTGAAAGTTG4700.1207586727851061No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTG4550.11690467258983675No Hit
GCTGCGTTCTTCATCGAGCTTTAGGCAAAATGTTTTAGGCATCTGACTAA4540.11664773924348545No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGCTAGTTA4500.1156200058580803No Hit
GCTGCGTTCTTCATCGCTGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT4230.10868280550659548No Hit
GCTGCGTTCTTCATCGATGCTAGGAAGAAACTGCATCAACTAACGAGCAA4220.10842587216024419No Hit
GCTGCGTTCTTCATCGGTATAGGAGCCTAGATATCCATCGTTGAAAGTTT4100.1053426720040287No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)