FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001994107

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001994107
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences306629
Sequences flagged as poor quality0
Sequence length35-301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG10591034.540111992016406No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTCAAGGGCCAGC7581824.726297903981685No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGGCCA6983422.774753855636618No Hit
TCCGTAGGTGAACCTGCTCCAGAAACTGAAGTTTGAAATGGAGCTCCTAA25970.8469518538690077No Hit
TCCGTAGGTGAACCTGCCCCTGCCAGGGTTTCAGCAGGGCCAAGCTGGAA13940.45462105671674896No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTCAAGGGCCAGC11750.38319924077631273No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG11400.37178479530638003No Hit
TCCGTAGGTGAACCTGCGGATGCATCGATGAAGAACGCAGC9900.32286574329238266No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTTGGGCAGGCCA9840.32090898121182276No Hit
TCCGTAGGTGAACCTGCGAGGACAATGGCCCATGACATTTCAGTGGCTGC9070.29579720117797076No Hit
TCCGTAGGTGAACCTGCCACAGCGGATTAGCGAGGCGGAACAGAGGGGAC8730.28470888272146466No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAAGTATGAAGCCGACTTGATCGT7510.24492138708341352No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTCAAGGTCCAGC6720.21915735302270822No Hit
TCCGTAGGTGAACCTGCCGAAGGATCATTAGTGAAGATTCAAGGGCCAGC5870.19143655688144307No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGCCTATACTTTTG4670.15230131527024515No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATCCTTTTG4470.14577877500171216No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGTCCA4450.14512652097485887No Hit
TCCGTAGGTGAACCTGCCGAAGGATCATTAGTGAAGATTTGGGCAGGCCA4350.14186525084059237No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTT4190.136647218625766No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGGCCC3970.12947242433037973No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACCTTTG3870.12621115419611323No Hit
TCCGTAGGTGAACCTGCCTGAGGGTGGCCACATTCCCACACCCCATGTGT3380.11023093053820741No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTTGGCAGGCCA3110.10142550117568788No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)