FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001994108

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001994108
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences306629
Sequences flagged as poor quality0
Sequence length35-301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG14161846.1854553874552No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA10308733.61945543311298No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTG38811.2656989391088254No Hit
GCTGCGTTCTTCATCGCTGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG38391.252001604544906No Hit
GCTGCGTTCTTCATCGATGCTCCCTGCCCTGACCACCAGGCCATGCATGG24990.8149914065531961No Hit
GCTGCGTTCTTCATCGCTGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA24130.7869444833985044No Hit
GCTGCGTTCTTCATCGATGTGAGAACCAAGAGATCCGTTGTCGAAAGTTG21150.6897586333973629No Hit
GCTGCGTTCTTCATCGATGCCTGGTGGAAGTGGGCTTACTCTGCATCCTG11700.3815686057091795No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGCAAGTTG10160.33134504564147554No Hit
GCTGCGTTCTTCATCGATGCATCCGCAGGTTCACCTACGGA8600.2804692315469183No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAACGTTG8580.27981697752006496No Hit
GCTGCGTTCTTGCTGCGGTTATTGGGTCAGGGCTGGGGCAAGGATCTAAG8330.27166380218439873No Hit
GCTGCGTTCTTCATCGATGCCGGGTGAGAGGAGAACGCGGCCTGGGGCGC8090.26383675386215916No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTGATAGTTA7840.2556835785264929No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGCGATCCGTTGTCGAAAGTTG7060.2302456714792143No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAATAAAATACTGGCAAACCG6800.2217663691301214No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGCTCCGTTGTCGAAAGTTG6720.21915735302270822No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGCGATCCGTTGTTGATAGTTA5750.18752303272032325No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG5520.18002211141151034No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATCGTTA5280.17219506308927074No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTCGATAGTTA5030.1640418877536045No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGCTA4380.14284363188087232No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTCG3860.12588502718268657No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGCTGTTGATAGTTA3810.12425439211555332No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGCTAGTTA3790.12360213808870002No Hit
GCTGCGTTCTTCATCGGTGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA3790.12360213808870002No Hit
GCTGCGTTCTTCATCGATGTGAGAACCAAGAGATCCGTTGTCGATAGTTG3750.12229763003499343No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGCTGTCGAAAGTTG3560.1161012167798871No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTCG3430.11186156560534066No Hit
GCTGCGTTCTTCATCGGTGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG3240.10566515235023433No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTCA3190.10403451728310108No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)