FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001994109

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001994109
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences245530
Sequences flagged as poor quality0
Sequence length35-301
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGGCCA13465654.84299270964852No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG4272417.400724962326397No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTCAAGGGCCAGC194037.902496639921802No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGCCCGCCAGGA67512.7495621716287215No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTTGGGCAGGCCA18440.7510283875697471No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGGCCC7370.30016698570439454No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG7330.29853785688103285No Hit
TCCGTAGGTGAACCTGCCGAAGGATCATTAGTGAAGATTTGGGCAGGCCA6910.28143200423573494No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGTCCA6770.275730053353969No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGCGAAGATTTGGGCAGGCCA6340.25821691850283063No Hit
TCCGTAGGTGAACCTGCCACAGCGGATTAGCGAGGCGGAACAGAGGGGAC6320.25740235409114975No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTTGGCAGGCCA6000.2443693235042561No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGCAGATTTGGGCAGGCCA5780.23540911497576672No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCCGGCCA5400.21993239115383048No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGATGATTTGGGCAGGCCA4440.18083329939314952No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATCTGGGCAGGCCA4020.16372744674785158No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGGCAA3530.14377061866167065No Hit
TCCGTAGGTGAACCTGCTCCAGAAACTGAAGTTTGAAATGGAGCTCCTAA3350.13643953895654298No Hit
TCCGTAGGTGAACCTGCAGAAGGCTCATTAGTGAAGATTTGGGCAGGCCA3200.1303303058689366No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTAGGCAGGCCA3010.12259194395796848No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGAGCAGGCCA3000.12218466175212805No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTCAAGGGCCAGC2840.11566814645868123No Hit
TCCGTAGGTGAACCTGCGATTGGATCATTACTAAATGATGGTAGTATCGA2660.10833706675355355No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)