FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001994110

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001994110
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences245530
Sequences flagged as poor quality0
Sequence length35-301
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG14623859.560135217692334No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA3917015.953244002769518No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG60432.4612063698936995No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTG39731.618132203804016No Hit
GCTGCGTTCTTCATCGCTGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG37431.5244572964607177No Hit
GCTGCGTTCTTCATCGATGGTAGAACCAAGAGATCCGTTGTCGAAAGTTG30591.2458762676658657No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA22940.9343053801979392No Hit
GCTGCGTTCTTCATCGATGTGAGAACCAAGAGATCCGTTGTCGAAAGTTG21470.8744348959393964No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGCAAGTTG15990.6512442471388425No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGCGATCCGTTGTCGAAAGTTG10080.41054046348715023No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAACGTTG9330.3799942980491182No Hit
GCTGCGTTCTTCATCGCTGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA9170.3734777827556714No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGCTCCGTTGTCGAAAGTTG7030.2863193907058201No Hit
GCTGCGTTCTTCATCGATGCCGGGTGAGAGGAGAACGCGGCCTGGGGCGC5950.24233291247505395No Hit
GCTGCGTTCTTCATCGGTGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG4280.1743167840997027No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG4260.17350221968802182No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTCG4010.16332016454201115No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGCTGTCGAAAGTTG3790.15435995601352176No Hit
GCTGCGTTCTTCATCGATGTGAGAACCAAGAGATCCGTTGTCGATAGTTG3460.14091964322078768No Hit
GCCGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG3450.14051236101494724No Hit
GCTGCGTTCTTCATCGATGCTCCCTGCCCTGACCACCAGGCCATGCATGG3230.13155215248645788No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTCG3050.1242210727813302No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTCGATAGTTA2710.11037347778275568No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGCCGAAAGTTG2670.10874434895939396No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGCTGATAGTTA2660.10833706675355355No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTAAAAGTTG2590.10548609131267055No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGCGATCCGTTGTTGATAGTTA2470.10059870484258543No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)