FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001994111

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001994111
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences428382
Sequences flagged as poor quality0
Sequence length35-301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAGGGATCATTACAAGAACGCCCGGGCTTCGG15250635.60046874051665No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG9832622.952878505632825No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACACAAATATGAAGGCGGGCT4943111.539000238105244No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAATGAATTTTAGGACTCTCTT421589.841216484352751No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGGCCA129733.0283718737015093No Hit
TCCGTAGGTGAACCTGCGGATGGATCATTAACACTATCTAAAAACAACTT80751.8849998365944414No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCAGAACGCCCGGGCTTCGG54201.2652258965129253No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCCAAATATGAAGGCGGGCT18290.42695538094504437No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACACAAATATGAAGGCGTGCT6100.14239627248577205No Hit
TCCGTAGGTGAACCTGCCACAGCGGATTAGCGAGGCGGAACAGAGGGGAC5320.12418822452857495No Hit
TCCGTAGGTGAACCTGCCCATTGTGCATCGATGAAGAACGCAGC4940.11531763706224818No Hit
TCCGTAGGTGAACCTGCGGATGGATCATTAACACTATCTAAAAACCACTT4500.10504643052229085No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)