FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001994112

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001994112
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences428382
Sequences flagged as poor quality0
Sequence length35-301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT14697034.308164208580195No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA9100521.24388979929129No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTG4944711.54273522230159No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTGAAAGTTT400519.349365752996158No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG126122.944101292771405No Hit
GCTGCGTTCTTCATCGTTACGAGAGCCAAGATATCCGTTGTTAAGAGTTG64481.505198631128292No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA52661.2292766736230747No Hit
GCTGCGTTCTTCATCGCTGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT36630.8550779444514476No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGCTAAAAGTTT21840.5098253428015183No Hit
GCTGCGTTCTTCATCGCTGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA21250.49605258857748463No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAACGTTT14650.3419844904781246No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGCGATCCGTTGTTAAAAGTTT11840.2763888305297608No Hit
GCTGCGTTCTTCATCGCTGCCAGAACCAAGAGATCCGTTGTTGAAAGTTG11510.2686854256247928No Hit
GCTGCGTTCTTCATCGCTGCGAGAGCCAAGAGATCCGTTGCTGAAAGTTT9460.22083094060908254No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCAAAAGTTT9200.21476159129001685No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTACAAGTTT8260.19281855913647167No Hit
GCTGCGTTCTTCATCGATACGAGAGCCAAGATATCCGTTGTTAAGAGTTG7910.18464828120696014No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGCTGAAAGTTG7570.1767114397897204No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGCTGTTAAAAGTTT6950.16223837602887142No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGCTCCGTTGTTAAAAGTTT6370.1486990583171095No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGCTGATAGTTA6020.14052878038759797No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGCGATCCGTTGTTGATAGTTA5570.1300241373353689No Hit
GCTGCGTTCTTCATCGATGCCGGGTGAGAGGAGAACGCGGCCTGGGGCGC5130.11975293079541156No Hit
GCTGCGTTCTTCATCGATGCACAATGGGCAGGTTCACCTACGGA4690.10948172425545426No Hit
GCTGCGTTCTTCATCGGTGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT4590.10714735913273667No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATCGTTA4410.10294550191184504No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAACGTTG4400.10271206539957328No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)