FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001994113

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001994113
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences218528
Sequences flagged as poor quality0
Sequence length35-301
%GC47

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG10058546.02842656318641No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACAAAAAATGAACTAATAAC2886713.209748865133989No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGGCCA2769112.67160272367843No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG168607.715258456582223No Hit
TCCGTAGGTGAACCTGCCACAGCGGATTAGCGAGGCGGAACAGAGGGGAC84993.889204129447943No Hit
TCCGTAGGTGAACCTGCTAAAGGAGTGCAGGAGAGGCTGAGATAAGAAGC26391.2076255674330063No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACAAAAAATGAACTACTAAC15080.6900717528188607No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTT4560.20866891199297116No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGCCTATACTTTTG3750.1716027236784302No Hit
TCCGTAGGTGAACCTGCCCGCATCGATGAAGAACGCAGC3530.16153536388929565No Hit
TCCGTAGGTGAACCTGCGAGGACAATGGCCCATGACATTTCAGTGGCTGC3510.1606201493630107No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGCTTTGACTATACTTTTG3370.15421364767901596No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTTGGGCAGGCCA3200.1464343242055938No Hit
TCCGTAGGTGAACCTGCGAGGTGCTTCTAGTGTTACCTTTACAGCATCGATGAAGAACGCAGC3140.1436886806267389No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATCCTTTTG3140.1436886806267389No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACCTTTG3030.13865500073217163No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGTCCA2620.11989310294332992No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAGGGATGAAGCCCACTTGATCAT2300.10524967052277054No Hit
TCCGTAGGTGAACCTGCGGATGGCCGGGTACTCCTGCAGGGTGGCGCACA2220.1015888124176307No Hit
TCCGTAGGTGAACCTGCGGAAGGCTCATTATTGATTTGACTATACTTTTG2210.10113120515448822No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATCGATTTGACTATACTTTTG2200.10067359789134574No Hit
TCCGTAGGTGAACCTGCCACAGCGGCTTAGCGAGGCGGAACAGAGGGGAC2200.10067359789134574No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)