FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001994114

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001994114
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences218528
Sequences flagged as poor quality0
Sequence length35-301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA9455043.26676673012154No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG2644412.100966466539758No Hit
GCTGCGTTCTTCATCGTTGTGGGAACCTAGACATCCATCGTTGAAAATTT166417.615042465954019No Hit
GCTGCGTTCTTCATCGATGTGGGAACCTAGACATCCATCGTTGAAAATTT90014.118922975545468No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG89324.087348074388637No Hit
GCTGCGTTCTTCATCGATGCCGGGTGAGAGGAGAACGCGGCCTGGGGCGC80413.6796200029286865No Hit
GCTGCGTTCTTCATCGATGCCAGTGCTTCTTATCTCAGCCTCTCCTGCAC25281.156831161224191No Hit
GCTGCGTTCTTCATCGCTGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA22781.0424293454385707No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA16540.7568824132376629No Hit
GCTGCGTTCTTCATCGCTGTGGGAACCTAGACATCCATCGTTGAAAATTT11520.5271635671401377No Hit
GCTGCGTTCTTCATCGACGCACGAGCCGAGTGATCCACCGCTAAGAGTCG11080.5070288475618685No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTG7810.3573912725142773No Hit
GCTGCGTTCTTCATCGCTGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG6890.3152914043051691No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGCTGATAGTTA6710.3070544735686045No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGCGTCG6350.2905806120954752No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGCGATCCGTTGTTGATAGTTA5830.2667850344120662No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATCGTTA5720.2617513545174989No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTCGATAGTTA5670.2594633182017865No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGGGATCCACCGCTAAGAGTCG5510.2521416019915068No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGCTA4760.21782105725582077No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCCCCGCTAAGAGTCG4690.21461780641382341No Hit
GCTGCGTTCTTCATCGGTGTGGGAACCTAGACATCCATCGTTGAAAATTT4390.200889588519549No Hit
GCTGCGTTCTTCATCGATGTGGGAACCTAGACATCCCTCGTTGAAAATTT4230.19356787230926928No Hit
GCTGCGTTCTTCATCGGTGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA3960.18121247620442232No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGCTGTTGATAGTTA3900.17846683262556742No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGCGATCCACCGCTAAGAGTCG3890.17800922536242494No Hit
GCTGCGTTCTTCATCGATGTGAGAACCAAGAGATCCGTTGTCGAAAGTTG3780.17297554546785765No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGCTAGTTA3770.17251793820471517No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGGGATCCCCCGCTAAGAGTCG3570.16336579294186557No Hit
GCTGCGTTCTTCATCGTTGTGGGAACCTAGACATCCATCGTTGAAACTTT3540.16199297115243813No Hit
GCTGCGTTCTTCATCGATGCGGGCAGGTTCACCTACGGA3500.16016254209986822No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGCAAGTTG3220.14734953873187875No Hit
GCTGCGTTCTTCATCGATGCTGTAAAGGTAACACTAGAAGCACCTCGCAGGTTCACCTACGGA3170.14506150241616636No Hit
GCTGCGTTCTTGCTGCGGTTATTGGGTCAGGGCTGGGGCAAGGATCTAAG3120.14277346610045394No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTCA3110.14231585883731146No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAACGTTG3050.1395702152584566No Hit
GCTGCGTTCTTCATCGCTGCACGAGCCGAGTGATCCACCGCTAAGAGTCG2980.1363669644164592No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGCTCCGTTGTTGATAGTTA2630.1203507102064724No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTACGAGTCG2500.11440181578562016No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)