FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001994115

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001994115
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences473241
Sequences flagged as poor quality0
Sequence length35-301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAATATAGGACGTCCAAC8533518.03203864415805No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGATGCCTAACATCAAACG6918414.619189799700363No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG6734414.23038156034663No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG6157213.01070701820003No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACATTAGAAGTATCTTTTATT438989.276034832146834No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAAGTGACCCCGGTTTACCA426419.010419638197028No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGACACCTAATCCTATATACCC254205.371470350202117No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGAATACGAATTGGTTCTG102902.174367816820605No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGATGCCTAACATCCAACG42270.8932024063849076No Hit
TCCGTAGGTGAACCTGCAGATGGATCATTAACACATCCACATAAACCTAA21690.45832884302078647No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCAGTGACCCCGGTTTACCA13360.28230859118292795No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCTTAGAAGTATCTTTTATT12220.25821938504905534No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAAGTATGAAGCCGACTTGATTGT11030.23307363478650414No Hit
TCCGTAGGTGAACCTGCCTTGCATCCTGGGGTGAAGCCAACTTGATCGTG9400.198630296191581No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)