FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001994116

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001994116
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences473241
Sequences flagged as poor quality0
Sequence length35-301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTT8214017.35690694593241No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG7143215.094212039954272No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA6384313.49058936144586No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT425698.995205402744057No Hit
GCTGCGTTCTTCATCGATGCGGGAACCAAGATATCCATTGCCTAGAGTTG412448.715221208644222No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG398218.414528749622285No Hit
GCTGCGTTCTTCATCGATGCATGAGCCAAGATATCCACTGTTAAGAGTTG229054.84002865347677No Hit
GCTGCGTTCTTCATCGATGGGAGAGCCAAGAGATCCGTTGCTGAAAGTTG95252.012716565132776No Hit
GCTGCGTTCTTCATCGACGCACGAGCCGAGTGATCCACCGCTAAGAGTCG47050.9942080250865838No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGGGATCCACCGCTAAGAGTCG26830.5669415794489489No Hit
GCTGCGTTCTTCATCGCTGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTT20180.42642121033469205No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGCGATCCACCGCTAAGAGTCG19940.42134979851703463No Hit
GCTGCGTTCTTCATCGCTGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG18060.38162373927871845No Hit
GCTGCGTTCTTCATCGTTACGAAAGCCGAGATATCCATCGCTGAGGTTTT17920.37866541571841833No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA17140.3621833273110318No Hit
GCTGCGTTCTTCATCGCTGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA15350.32435904750433714No Hit
GCTGCGTTCTTCATCGATGCGGGAACCAAGATATCCCTTGCCTAGAGTTG14750.31168051796019364No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCCCCGCTAAGAGTCG13260.28019550292557066No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGGGATCCCCCGCTAAGAGTCG12780.2700526792902559No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAATAAAATACTGGCAAACCG10870.22969269357473254No Hit
GCTGCGTTCTTCATCGCTGCACGAGCCGAGTGATCCACCGCTAAGAGTCG10720.2265230611886967No Hit
GCTGCGTTCTTCATCGCTGCGGGAACCAAGATATCCATTGCCTAGAGTTG10710.22631175236296094No Hit
GCTGCGTTCTTCATCGCTGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT10370.2191272522879463No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGCGTCG9980.21088620808425307No Hit
GCTGCGTTCTTCATCGATGCATGAGCCAAGATATCCCCTGTTAAGAGTTG9370.19799636971437384No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAACGTTT8520.1800351195268373No Hit
GCTGCGTTCTTCATCGATGCGAAAATCCTCAATAAAATATGCCAAACCAA8340.17623156066359424No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGCGATCCCCCGCTAAGAGTCG8250.1743297812319727No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGCTATCCGTTGCCGAGAGTCG6030.12741922191864188No Hit
GCTGCGTTCTTCATCGATGCGGGAACCAAGATATCCATTGCCTAGCGTTG5810.12277042775245593No Hit
GCTGCGTTCTTCATCGCTGCATGAGCCAAGATATCCACTGTTAAGAGTTG5430.11474069237449841No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGCGATCCGTTGTTGAAAGTTT4780.1010056187016763No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)