FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001994117

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001994117
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences255620
Sequences flagged as poor quality0
Sequence length35-301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAAGGATCATTACAGAGTTCATGCCCAAAAGG5216620.40763633518504No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTCACACATCCAATCAAACCTAA4354617.035443236053517No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTTACACATCCAATCAAACCTAA3010511.777247476723261No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAAAATTAATTTATTACATTGT210118.2196228777091No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAAGAACGCCCGGGCTTCGG138705.426023002894922No Hit
TCCGTAGGTGAACCTGCCACAGCGGATTAGCGAGGCGGAACAGAGGGGAC123314.82395743682028No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGTACCGTTTTCGGGGGAT107424.202331585947892No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTCCCAAA101903.9863860417807686No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTCATAATTTATGAAATGTTCTT77913.0478835771848836No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACAGAGTTCATGCCCACAAGG31611.2366012049135435No Hit
TCCGTAGGTGAACCTGCGGACTGCATCGATGAAGAACGCAGC20730.8109694077145763No Hit
TCCGTAGGTGAACCTGCGGAGCATCGATGAAGAACGCAGC20320.7949299741804241No Hit
TCCGTAGGTGAACCTGACCGTGGTCCGTTACAGCCCAAAGCTTCGAGCAG18330.7170800406853923No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG13390.5238244268836554No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTTCATGCCCAAAAGG10890.4260230028949222No Hit
TCCGTAGGTGAACCTGCGGTTGGATCATTAATAAATCTAGAAGGTCTTAA10140.3966825756983022No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACATTAATTTATTACATTGT7340.2871449808309209No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTTCATGCCCACAAGG6490.2538924966747516No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAAAATTAATTTATTACCTTGT6160.2409827087082388No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCAGAACGCCCGGGCTTCGG4650.18191064861904388No Hit
TCCGTAGGTGAACCTGCGGAATCTGATGATAAAGCTTTAACATCGTAAAT3770.14748454737500977No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACAGAGTTCATGCCCCAAAGG3460.13535717080040685No Hit
TCCGTAGGTGAACCTGCGAGGTGCTTCTAGTGTTACCTTTACAGCATCGATGAAGAACGCAGC3310.12948908536108286No Hit
TCCGTAGGTGAACCTGCGAGGACAATGGCCCATGACATTTCAGTGGCTGC3300.12909787966512792No Hit
TCCGTAGGTGAACCTGCCACAGCGGCTTAGCGAGGCGGAACAGAGGGGAC3140.122838588529849No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)