FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001994118

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001994118
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences255620
Sequences flagged as poor quality0
Sequence length35-301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT6366024.90415460449104No Hit
GCTGCGTTCTTCATCGATATGGGAGCCAAGACATCCACTGTTGAAAATTT6047123.656599640090757No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT206408.07448556450982No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT134975.280103278303732No Hit
GCTGCGTTCTTCATCGATGCCGGGTGAGAGGAGAACGCGGCCTGGGGCGC116844.570847351537438No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGGGGTCCACCGTTAGAAGTTG86233.3733667162193877No Hit
GCTGCGTTCTTCATCGGTATAGGAGCCTAGATATCCATCGTTGAAAATTT70272.749002425475315No Hit
GCTGCGTTCTTCATCGATGTGGGAGCCAAGACATCCACTGTTGAAAATTT37811.479148736405602No Hit
GCTGCGTTCTTCATCGATATGGGAGCCAAGACATCCCCTGTTGAAAATTT23040.901337923480166No Hit
GCTGCGTTCTTCATCGATGCTCCGCAGGTTCACCTACGGA20380.7972772083561538No Hit
GCTGCGTTCTTCATCGATGCAGTCCGCAGGTTCACCTACGGA19090.7468116735779673No Hit
GCTGCGTTCTTCATCGATGCATGAGAAACATACAGTGTGTGCCCAGGGAG17800.6963461387997809No Hit
GCTGCGTTCTTCATCGCTGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT15540.6079336515139661No Hit
GCTGCGTTCTTCATCGATATGGGAGCCAAGACATCCACTGTTGAAACTTT14940.5844613097566701No Hit
GCTGCGTTCTTCATCGCTATGGGAGCCAAGACATCCACTGTTGAAAATTT13840.5414286832016274No Hit
GCTGCGTTCTTCATCGGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTT10370.4056803067052656No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCTAGAGATCCGTTGTCAAAAGTTG9530.37281902824505125No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGCTGAAAGTTT9330.3649949143259526No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGGGGTCCCCCGTTAGAAGTTG7750.30318441436507315No Hit
GCTGCGTTCTTCATCGATATGGGAGCCAAGACATCCACTGTTGAAAGTTT6780.2652374618574446No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG5950.2327673890931852No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGCGATCCGTTGTTGAAAGTTT5640.22064001251858226No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAACGTTT5210.20381816759252017No Hit
GCTGCGTTCTTCATCGATATGGGAGCCAAGACATCCACTGTTGAACATTT4570.17878100305140443No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCGAAAGTTT4570.17878100305140443No Hit
GCTGCGTTCTTCATCGCTGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT4440.1736953290039903No Hit
GCTGCGTTCTTCATCGATATGGGAGCCAAGACATCCACTGTTGAAAATCT4250.16626242078084658No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGCTT4170.1631327752132071No Hit
GCTGCGTTCTTCATCGATACGGGAGCCAAGACATCCACTGTTGAAAATTT3640.14239887332759563No Hit
GCTGCGTTCTTCATCGATATGGGAGCCAAGACATCCACTGTTGCAAATTT3550.13887802206400127No Hit
GCTGCGTTCTTATCAGACGATGGCAAATTAATGATTGATGATCAACAATA3510.1373131992801815No Hit
GCTGCGTTCTTCATCGCTGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT3440.13457475940849697No Hit
GCTGCGTTCTTCATCGATGCTGTAAAGGTAACACTAGAAGCACCTCGCAGGTTCACCTACGGA3300.12909787966512792No Hit
GCTGCGTTCTTCATCGATATGTGAGCCAAGACATCCACTGTTGAAAATTT3190.12479461700962366No Hit
GCTGCGTTCTTGCTGCGGTTATTGGGTCAGGGCTGGGGCAAGGATCTAAG3160.12362099992175887No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGCAAGTTT2930.1146232689147954No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGCTGAAAGTCT2790.10914638917142634No Hit
GCTGCGTTCTTCATCGCTGCCGGGTGAGAGGAGAACGCGGCCTGGGGCGC2750.1075815663876066No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGGGGTCCACCGCTAGAAGTTG2610.10210468664423754No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGCTCCGTTGTTGAAAGTTT2560.10014865816446288No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)