FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001994119

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001994119
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401223
Sequences flagged as poor quality0
Sequence length35-301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGATGCCTAAACATCAAAC17271943.04813034148092No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGATGCCTAACATCAAACG5760614.35760163300708No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTCACACATCCAATCAAACCTAA4604711.476660111708451No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGCCTAGCAGAA262236.535766892725492No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGTACCGTTTTCGGGGGAT158693.9551571071449043No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGGCCA115942.8896648497219752No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATCGATTCGTATTAACCTGCC76281.9011871203794397No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTCACACATCCAATCAAACGTAA55471.3825229361228046No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGATGCCTAACATCCAACG34930.8705881766498929No Hit
TCCGTAGGTGAACCTGCATCGATGAAGAACGCAGC10040.25023490677254295No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGATGCCTCAACATCAAAC8530.2125999755746804No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGATGCCTAAACATCTAAC5760.14356106205277366No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGCTGCCTAAACATCAAAC5490.13683163726904987No Hit
TCCGTAGGTGAACCTGCGGAAGGCTCATTGTCGATGCCTAAACATCAAAC5240.1306006883952316No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGATGCCTAAAAATCAAAC4270.10642460676481658No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG4040.10069213380090375No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)