FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001994121

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001994121
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences306035
Sequences flagged as poor quality0
Sequence length35-301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG13350243.62311500318591No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGATGCCTAAACATCAAAC5664018.50768702926136No Hit
TCCGTAGGTGAACCTGCTGAAGGATCATTATTGATTTGACTATACTTTTG275689.00811998627608No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACACAAATATGAAGGCGGGCT256458.379760484911856No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTCACACATCCAATCAAACCTAA233857.641282859803618No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCCAAATATGAAGGCGGGCT9360.3058473703988106No Hit
TCCGTAGGTGAACCTGCTCCAGAAACTGAAGTTTGAAATGGAGCTCCTAA8370.2734981292989364No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTT5620.1836391262437303No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGCCTATACTTTTG5350.1748166059437646No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATCCTTTTG4310.1408335647883412No Hit
TCCGTAGGTGAACCTGCATCGATGAAGAACGCAGC4230.1382194846994625No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACCTTTG4070.13299132452170503No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGCTTTGACTATACTTTTG3870.12645612429950823No Hit
TCCGTAGGTGAACCTGCCCAGCATCGATGAAGAACGCAGC3380.11044488375512604No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACACAAATATGAAGGCGTGCT3370.11011812374401621No Hit
TCCGTAGGTGAACCTGCGGAAGGCTCATTATTGATTTGACTATACTTTTG3100.10129560344405052No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)