FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001994122

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001994122
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences306035
Sequences flagged as poor quality0
Sequence length35-301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA14923548.76403025797703No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG5371817.552894276798405No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTG253838.294149362001079No Hit
GCTGCGTTCTTCATCGATATGGGAGCCAAGACATCCACTGTTGAAAATTT191256.249285212475697No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA67132.193539954580358No Hit
GCTGCGTTCTTCATCGCTGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA36501.1926740405509173No Hit
GCTGCGTTCTTCATCGCTGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG13170.4303429346316598No Hit
GCTGCGTTCTTCATCGATGTGGGAGCCAAGACATCCACTGTTGAAAATTT10750.3512670119430784No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGCGATCCGTTGTTGATAGTTA10100.33002761122093877No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGCTGATAGTTA9950.3251262110542912No Hit
GCTGCGTTCTTCATCGATGCTCCCTGCCCTGACCACCAGGCCATGCATGG7950.2597742088323231No Hit
GCTGCGTTCTTCATCGATATGGGAGCCAAGACATCCCCTGTTGAAAATTT7560.24703056839903934No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATCGTTA7210.23559396801019492No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTCGATAGTTA7070.23101932785465715No Hit
GCTGCGTTCTTCATCGCTGCCAGAACCAAGAGATCCGTTGTTGAAAGTTG6460.21108696717695688No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGCTA6310.2061855670103093No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGCTGTTGATAGTTA5810.18984756645481726No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGCTAGTTA5370.1754701259659843No Hit
GCTGCGTTCTTCATCGATATGGGAGCCAAGACATCCACTGTTGAAACTTT5090.16632084565490876No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTCA4970.16239972552159065No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGCTATCCGTTGCCGAGAGTCG4870.15913212541049226No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGCGTCG4820.15749832535494307No Hit
GCTGCGTTCTTCATCGCTATGGGAGCCAAGACATCCACTGTTGAAAATTT4810.15717156534383322No Hit
GCTGCGTTCTTCATCGATGCAGGTTCACCTACGGA4120.13462512457725423No Hit
GCTGCGTTCTTCATCGGTGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA4110.13429836456614438No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGCTGAAAGTTG4050.13233780449948537No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGCTCCGTTGTTGATAGTTA3840.12547584426617872No Hit
GCTGCGTTCTTCATCGATGCTGGGCAGGTTCACCTACGGA3520.1150195239106638No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)