FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001994123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001994123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences312168
Sequences flagged as poor quality0
Sequence length35-301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAAGGATCATTCACACATCCAATCAAACCTAA9152929.320430024858407No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG8175026.187821942031213No Hit
TCCGTAGGTGAACCTGCTGAAGGATCATTAACACTAATCCAAAAAACTCA4009212.843084492965327No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGGCCA295159.454844827144358No Hit
TCCGTAGGTGAACCTGCAAAAATTGAGCAATGACCGCATCAGGGTGGGGG36841.1801337741216267No Hit
TCCGTAGGTGAACCTGCATCCCAGGGATTAAGCACACTTGATCTTGGTGG25280.8098203531431792No Hit
TCCGTAGGTGAACCTGCCCCCCAGCACAGGGCCATGCTGCACCAAGGTCA18800.6022398195843264No Hit
TCCGTAGGTGAACCTGCTGAAGGATCATTAACACTAATCCAAAAACCTCA17620.564439660695523No Hit
TCCGTAGGTGAACCTGCGAGGACAATGGCCCATGACATTTCAGTGGCTGC16740.5362497116937034No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG13960.44719510007431895No Hit
TCCGTAGGTGAACCTGCTGAAGGATCATTCACACATCCAATCAAACCTAA11050.3539760641705748No Hit
TCCGTAGGTGAACCTGCTAGCCCAGTGCCCAGGTGAACCCGCTCGCCCGG9920.317777606929602No Hit
TCCGTAGGTGAACCTGCGCATCGATGAAGAACGCAGC9570.3065656953947874No Hit
TCCGTAGGTGAACCTGCCCATTGGCATCGATGAAGAACGCAGC9250.29631480484867123No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8890.2847825529842905No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACACTAATCCAAAAAACTCA7900.2530688603572435No Hit
TCCGTAGGTGAACCTGCGAGGTGCTTCTAGTGTTACCTTTACAGCATCGATGAAGAACGCAGC7270.2328874195945773No Hit
TCCGTAGGTGAACCTGCCACAGCGGATTAGCGAGGCGGAACAGAGGGGAC6500.2082212142179852No Hit
TCCGTAGGTGAACCTGCGGAATCTGATGATAAAGCTTTAACATCGTAAAT5350.17138207631788013No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTTGGGCAGGCCA5320.17042105532918173No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTCACACATCCCATCAAACCTAA5210.16689731170395428No Hit
TCCGTAGGTGAACCTGCGCCAGTCACTCAATCTCTTCCTCAATGACTCAA5070.16241254709002845No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAAGTATGAAGCCGACTTGATTGT4890.15664642115783808No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTCACACATCCAATCAAACCTAC4380.1403090643499654No Hit
TCCGTAGGTGAACCTGCGAAAATTGAGCAATGACCGCATCAGGGTGGGGG4220.1351836190769073No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTT4170.13358191742907666No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTCACACATCCACTCAAACCTAA3980.12749545116732017No Hit
TCCGTAGGTGAACCTGCTGAAGGATCATTAACACTAATCCAAAACACTCA3720.11916660259860075No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTCACACATCCAATCAACCCTAA3510.11243945567771199No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGCCTATACTTTTG3360.10763435073422004No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)