FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001994124

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001994124
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences312168
Sequences flagged as poor quality0
Sequence length35-301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA6223619.936700750877733No Hit
GCTGCGTTCTTCATCGATATGGGAGCCAAGACATCCACTGTTGAAAATTT4886915.654711565567258No Hit
GCTGCGTTCTTCATCGTTATGGGAACCAAGATATCCACCGTTGAGAATTT3209010.279721175777146No Hit
GCTGCGTTCTTCATCGATGTGGGAGCCAAGACATCCACTGTTGAAAATTT308459.880897465467314No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG285029.130340073293867No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA169625.433612670100715No Hit
GCTGCGTTCTTCATCCCGGTTTCAACTCCACTATAGGAACCCTGGAGGAC38661.2384357141026627No Hit
GCTGCGTTCTTCATCGATATGGGAACCAAGATATCCACCGTTGAGAATTT20920.6701519694523461No Hit
GCTGCGTTCTTCATCGATGCAAACATTCTCAATAAAATACTGGCAAACCG20750.664706183849722No Hit
GCTGCGTTCTTCATCGATATGGGAGCCAAGACATCCCCTGTTGAAAATTT17670.5660413623433536No Hit
GCTGCGTTCTTCATCGCTATGGGAACCAAGATATCCACCGTTGAGAATTT17200.5509853668537454No Hit
GCTGCGTTCTTCATCGATGCCCAGGTTGTTGTTGATGATTTTCAGAATGT15760.5048563593962225No Hit
GCTGCGTTCTTGCTGCGGTTATTGGGTCAGGGCTGGGGCAAGGATCTAAG15660.5016529561005613No Hit
GCTGCGTTCTTCATCGCTGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA14450.4628917762230593No Hit
GCTGCGTTCTTCATCGATGCGGGAGCCAAGACATCCACTGTTGAAAATTT13110.41996617206119785No Hit
GCTGCGTTCTTCATCGCTATGGGAGCCAAGACATCCACTGTTGAAAATTT11720.3754388662515056No Hit
GCTGCGTTCTTCATCGATATGGGAGCCAAGACATCCACTGTTGAAACTTT11690.3744778452628072No Hit
GCTGCGTTCTTCATCGATGTGGGAGCCAAGACATCCCCTGTTGAAAATTT11440.3664693370236539No Hit
GCTGCGTTCTTCATCGGTATGGGAACCAAGATATCCACCGTTGAGAATTT9550.3059250147356552No Hit
GCTGCGTTCTTCATCTTCATCCCCATCTGTCAATGGGACGGGACTCACCC9100.2915096999051793No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8810.2822198303477615No Hit
GCTGCGTTCTTCATCGATGCGCAGGTTCACCTACGGA8770.28093846902949693No Hit
GCTGCGTTCTTCATCGATGCCAATGGGCAGGTTCACCTACGGA8710.2790164270521001No Hit
GCTGCGTTCTTCATCGCTGTGGGAGCCAAGACATCCACTGTTGAAAATTT7740.2479434150841854No Hit
GCTGCGTTCTTCATCGTTATGGGAACCAAGATATCCACCGTTGAGACTTT7710.24698239409548703No Hit
GCTGCGTTCTTCATCGCTGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG7560.24217728915199507No Hit
GCTGCGTTCTTCATCGATGCTGTAAAGGTAACACTAGAAGCACCTCGCAGGTTCACCTACGGA7330.23480946157197405No Hit
GCTGCGTTCTTCATCGATGTGGGAGCCAAGACATCCACTGTTGAAACTTT7280.2332077599241434No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTG7180.2300043566284821No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAATAAAATACTGGCAAACCG6910.22135516773019656No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG6610.21174495784321265No Hit
GCTGCGTTCTTCATCGATGCCGGGTGAGAGGAGAACGCGGCCTGGGGCGC6320.2024550882857948No Hit
GCTGCGTTCTTCATCGTTATGGGAACCAAGATATCCACCGCTGAGAATTT6260.20053304630839805No Hit
GCTGCGTTCTTCATCGATTGGAGAACCAAGAGATCCGTTGTCGAAAGTTG5930.18996181543271573No Hit
GCTGCGTTCTTCATCGATATGGGAGCCAAGACATCCACTGTTGAAAGTTT5330.17074139565874785No Hit
GCTGCGTTCTTATCAGACGATGGCAAATTAATGATTGATGATCAACAATA5130.16433458906742524No Hit
GCTGCGTTCTTCATCGTTATGGGAACCAAGATATCCACCGTTGAGCATTT4890.15664642115783808No Hit
GCTGCGTTCTTCATCGATGTGGGAGCCAAGACATCCACTGTTGAAAGTTT4770.1528023372030445No Hit
GCTGCGTTCTTCATCGAGCACCTACTGTGTTCAGGCATCCTGCTGGGTGC4690.15023961456651547No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGCTGATAGTTA4330.13870736270213477No Hit
GCTGCGTTCTTCATCGATGTGAGAACCAAGAGATCCGTTGTCGAAAGTTG4230.13550395940647345No Hit
GCTGCGTTCTTCATCGTTATGGGAACCAAGATATCCCCCGTTGAGAATTT4160.1332615770995105No Hit
GCTGCGTTCTTCATCGCTGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA4110.13165987545167987No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGCGATCCGTTGTTGATAGTTA3920.12557340918992338No Hit
GCTGCGTTCTTCATCGATATGGGAGCCAAGACATCCACTGTTGAACATTT3780.12108864457599754No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTCGATAGTTA3540.1134004766664104No Hit
GCTGCGTTCTTCATCGCTATGGGAACCAAGATATCCCCCGTTGAGAATTT3230.10346992644986033No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATCGTTA3220.10314958612029419No Hit
GCTGCGTTCTTCATCGATATGGGAGCCAAGACATCCACTGTTGAAAATCT3150.10090720381333129No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)