FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001994125

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001994125
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences424606
Sequences flagged as poor quality0
Sequence length35-301
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGATGCCTAAACATCAAAC20644948.62131010866545No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG7782218.328050004003714No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGTGACCCTGACCAAAACA202444.767714069042831No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGATGCCTAACATCAAACG191804.517128820600745No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGATAAAACTATTATCTTA116592.7458396725434877No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGGCCA99842.3513563162084377No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTCATAATTTATGTAATGTTCTT76131.7929562935992425No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAATAAAACAATGAACTTTTAA67121.5807595747587173No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGTGACCCTGACCACAACA13460.31699975977729944No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG13070.3078147741671102No Hit
TCCGTAGGTGAACCTGCGAGGACAATGGCCCATGACATTTCAGTGGCTGC11950.2814373795942592No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGATGCCTAACATCCAACG11440.2694262445655502No Hit
TCCGTAGGTGAACCTGCATCGATGAAGAACGCAGC10530.24799461147510868No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGATGCCTCAACATCAAAC10500.24728807412047873No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGCTGCCTAAACATCAAAC7220.17003999001427206No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGATGCCTAAACATCTAAC6730.15849987988864972No Hit
TCCGTAGGTGAACCTGCGGAAGGCTCATTGTCGATGCCTAAACATCAAAC6400.15072796898772037No Hit
TCCGTAGGTGAACCTGCCCTGCATCGATGAAGAACGCAGC4830.11375251409542023No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGATGCCTAAACATCCAAC4690.11045533977381385No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGATGCCTAAACATCACAC4670.10998431487072721No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGATGCCTAAAAATCAAAC4660.1097488024191839No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGATGCCTAAACCTCAAAC4440.10456752848523101No Hit
TCCGTAGGTGACCCTGCGGAAGGATCATTGTCGATGCCTAAACATCAAAC4400.10362547867905778No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)