FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001994126

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001994126
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences424606
Sequences flagged as poor quality0
Sequence length35-301
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG24068156.683372349896146No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA7476517.608088439635804No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG112022.6382104821881933No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG96552.2738727196506883No Hit
GCTGCGTTCTTCATCGGTATAGGAGCCTAGATATCCATCGTTGAAAATTT70551.661540345638074No Hit
GCTGCGTTCTTCATCGCTGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG55371.3040324441953246No Hit
GCTGCGTTCTTCATCGTTATGGGAGCCTAGACATCCATCGTTGAAAATTT53851.268234551560741No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGCTATCCGTTGCCGAGAGTCG17900.4215672882625305No Hit
GCTGCGTTCTTCATCGCTGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA17340.4083785909761049No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA15690.36951903647145823No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGCGTCG15690.36951903647145823No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGATAGTCG13670.321945521259709No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATCTCCGTTGCCGAGAGTCG11910.2804953297880859No Hit
GCTGCGTTCTTCATCGGTGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG11120.26188984611616417No Hit
GCTGCGTTCTTGCTGCGGTTATTGGGTCAGGGCTGGGGCAAGGATCTAAG11020.259534721600731No Hit
GCTGCGTTCTTCATCGATGCAGGTTCACCTACGGA10550.24846563637819533No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGAGATCCGTTGCCGAGAGTCG7820.18417073710687085No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGCGAGTCG7730.18205112504298102No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG6450.15190553124543696No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGCTGCCGAGAGTCG5460.12858979854264896No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGCTGATAGTTA4970.1170496884170266No Hit
GCTGCGTTCTTCATCGATGCAGGGCAGGTTCACCTACGGA4870.11469456390159347No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGACATCCGTTGCCGAGAGTCG4830.11375251409542023No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAAAGTCG4710.11092636467690048No Hit
GCTGCGTTCTTCATCGATGCGAGAACCGAGATATCCGTTGCCGAGAGTCG4650.10951328996764058No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTCGATAGTTA4630.10904226506455396No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)