FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001994127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001994127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences367177
Sequences flagged as poor quality0
Sequence length35-301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAAGGATCATTACAGAGTTCATGCCCGAAAGG10914629.725718114152027No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAAGAACGCCCGGGCTTCGG5610115.279007127352747No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACACTAATCCAAACCTACTC4851313.212428883072741No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTCAAGGGCCAGC4813413.109208910144154No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTCCAAATTAAAACAGTGGGTAG148364.040558095959169No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG143353.9041116409796914No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACAGAGTTCATGCCCGCAAGG56201.5305969600492404No Hit
TCCGTAGGTGAACCTGCGTCTCAGTTCTCATACTGTAAACAAGTCTCCTT38151.039008434624173No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACACTTGGGTCTCGTGCCCG29910.8145935066738931No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTTCATGCCCGAAAGG21970.5983490251295697No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCAGAACGCCCGGGCTTCGG18650.5079294182369811No Hit
TCCGTAGGTGAACCTGCCACAGCGGATTAGCGAGGCGGAACAGAGGGGAC18290.4981248825498329No Hit
TCCGTAGGTGAACCTGCGGACTGCATCGATGAAGAACGCAGC13330.3630401686380138No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTTCATGCCCGCAAGG11670.31783036519171953No Hit
TCCGTAGGTGAACCTGCGTGCCTGTGGCTCACTCCAGGGGTGTGTTGCGT5490.14951916922900946No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTCCCAATTAAAACAGTGGGTAG5180.14107637460952074No Hit
TCCGTAGGTGAACCTGCGAGGACAATGGCCCATGACATTTCAGTGGCTGC4460.12146730323522442No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACAGAGTTCCTGCCCGAAAGG4200.11438624968339521No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACAGCGTTCATGCCCGAAAGG3810.10376466935565136No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)