FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001994128

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001994128
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences367177
Sequences flagged as poor quality0
Sequence length35-301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT11507031.339108931114968No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT5441514.819828039337976No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG4621012.585210947308791No Hit
GCTGCGTTCTTCATCGTTTTTGGAGCCTAGATATCCATCGTTGAAAATTT124863.40053979415922No Hit
GCTGCGTTCTTCATCGATGTAGGAACCTAGATATCCATCGTTGAGGATTT111103.025788652339335No Hit
GCTGCGTTCTTCATCGTTGTAGGAACCTAGATATCCATCGTTGAGTATTT104782.8536645813871786No Hit
GCTGCGTTCTTCATCGTTGTAGGAACCTAGATATCCATCGTTGAGGATTT94672.578320537506434No Hit
GCTGCGTTCTTCATCGATGTAGGAACCTAGATATCCATCGTTGAGTATTT81812.2280807349044194No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG77302.1052516906015355No Hit
GCTGCGTTCTTCATCGATGCATTAACATTGAACTCATGGCCCACAGCACC38241.04145956854596No Hit
GCTGCGTTCTTCATCGCTGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT27240.7418765336608775No Hit
GCTGCGTTCTTCATCGATGCCGGGTGAGAGGAGAACGCGGCCTGGGGCGC17230.4692559719154522No Hit
GCTGCGTTCTTCATCGTTGTGGGAACCAAGATATCCATTGCTAAAAGTTG15570.42404616846915794No Hit
GCTGCGTTCTTCATCGCTGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT13740.37420644539282144No Hit
GCTGCGTTCTTCATCGATGCAGTCCGCAGGTTCACCTACGGA12730.3466992758261002No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTG12580.34261405262312183No Hit
GCTGCGTTCTTCATCGCTGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG11530.31401749020227304No Hit
GCTGCGTTCTTCATCGACGCACGAGCCGAGTGATCCACCGCTAAGAGTCG11210.30530234736925244No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGCTGAAAGTTT10540.2870550170626156No Hit
GCTGCGTTCTTCATCGATGTGGGAACCAAGATATCCATTGCTAAAAGTTG10210.2780675260160631No Hit
GCTGCGTTCTTCATCGCTGTAGGAACCTAGATATCCATCGTTGAGGATTT8930.24320695468398076No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAACGTTT8430.22958954400738607No Hit
GCTGCGTTCTTCATCGATTTTGGAGCCTAGATATCCATCGTTGAAAATTT8250.224687276163812No Hit
GCTGCGTTCTTCATCGATGTAGGAACCTAGATATCCCTCGTTGAGGATTT7740.21079751727368545No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGCTAAAAGTTT7660.2086187315654303No Hit
GCTGCGTTCTTCATCGCTTTTGGAGCCTAGATATCCATCGTTGAAAATTT7430.20235472265419677No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGCTT7110.19363957982117616No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGCGATCCGTTGTTGAAAGTTT6540.17811573164985822No Hit
GCTGCGTTCTTCATCGATGTGAGAACCAAGAGATCCGTTGTCGAAAGTTG6340.17266876737922038No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCGAAAGTTT6190.16858354417624197No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGCGTCG5710.1555108299267111No Hit
GCTGCGTTCTTCATCGATGCCAAAGGGTTCAGGATTTCCTCCGCCCTCCC5370.14625099066662672No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGGGATCCACCGCTAAGAGTCG4850.13208888356296827No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAACGTTT4770.1299100978547131No Hit
GCTGCGTTCTTCATCGCTGTAGGAACCTAGATATCCATCGTTGAGTATTT4760.12963774964118124No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTCT4500.12255669608935202No Hit
GCTGCGTTCTTCATCGGTGTAGGAACCTAGATATCCATCGTTGAGGATTT4290.11683738360518225No Hit
GCTGCGTTCTTGCTGCGGTTATTGGGTCAGGGCTGGGGCAAGGATCTAAG4190.11411390146986332No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGCTCCGTTGTTGAAAGTTT4030.10975633005335302No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGCAAGTTG3780.10294762471505568No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGCGATCCGTTGTTAAAAGTTT3770.10267527650152379No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)