FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001994130

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001994130
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222748
Sequences flagged as poor quality0
Sequence length35-301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATATGGGAGCCAAGACATCCACTGTTGAAAATTT8032036.058685150932895No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA4491420.163592939106074No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCAAGAGATCCATTGTTAAAAGTTG186378.36685402338068No Hit
GCTGCGTTCTTCATCGATGCCAGGCACGTGGCCCATGCCCACGACGCCCA66122.9683768204428325No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAATAAAATACTGGCAAACCG65042.919891536624347No Hit
GCTGCGTTCTTCATCGATGTGGGAGCCAAGACATCCACTGTTGAAAATTT42831.9228006536534559No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG32061.4392946289080035No Hit
GCTGCGTTCTTCATCGATATGGGAGCCAAGACATCCCCTGTTGAAAATTT30211.3562411334781905No Hit
GCTGCGTTCTTCATCGATGCCGGGTGAGAGGAGAACGCGGCCTGGGGCGC22551.0123547686174512No Hit
GCTGCGTTCTTCATCGCTATGGGAGCCAAGACATCCACTGTTGAAAATTT20690.9288523353745038No Hit
GCTGCGTTCTTCATCGATATGGGAGCCAAGACATCCACTGTTGAAACTTT18790.8435541508790202No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA15480.694955734731625No Hit
GCTGCGTTCTTCATCGATGCAGGTTCACCTACGGA11180.5019124750839513No Hit
GCTGCGTTCTTCATCGCTGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA10890.48889327850306175No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCAAGAGATCCCTTGTTAAAAGTTG10460.46958895253829436No Hit
GCTGCGTTCTTCATCGATATGGGAGCCAAGACATCCACTGTTGAAAGTTT8740.39237164867922497No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8190.36768006895684807No Hit
GCTGCGTTCTTCATCGATGCGATCCTTCCGCAGGTTCACCTACGGA6670.29944152136046115No Hit
GCTGCGTTCTTCATCGATATGGGAGCCAAGACATCCACTGTTGAACATTT5830.2617307450571947No Hit
GCTGCGTTCTTCATCGATATGGGAGCCAAGACATCCACTGTTGAAAATCT4810.21593908811751394No Hit
GCTGCGTTCTTCATCGATGCTGTAAAGGTAACACTAGAAGCACCTCGCAGGTTCACCTACGGA4790.21504121249124572No Hit
GCTGCGTTCTTCATCGATATGTGAGCCAAGACATCCACTGTTGAAAATTT4670.2096539587336362No Hit
GCTGCGTTCTTCATCGATACGGGAGCCAAGACATCCACTGTTGAAAATTT4590.20606245622856323No Hit
GCTGCGTTCTTCATCGCTGCAAGAGCCAAGAGATCCATTGTTAAAAGTTG4320.19394113527394186No Hit
GCTGCGTTCTTCATCGATATGGGAGCCAAGACATCCACTGTTGCAAATTT4270.19169644620827123No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAATAAACTACTGGCAAACCG3770.169249555551565No Hit
GCTGCGTTCTTCATCGATGCGGGCAGGTTCACCTACGGA3560.1598218614757484No Hit
GCTGCGTTCTTCATCGATATGGGAGCCAAGACATCCACTGCTGAAAATTT3480.1562303589706754No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAAGAAAATACTGGCAAACCG3430.15398566990500476No Hit
GCTGCGTTCTTCATCGATATGTGAGCCAAGACATCCCCTGTTGAAAATTT3120.14006859769784688No Hit
GCTGCGTTCTTCATCGATGCCAGTGCTTCTTATCTCAGCCTCTCCTGCAC3100.13917072207157863No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGCTGATAGTTA3060.13737497081904215No Hit
GCTGCGTTCTTCATCGATATGGGAGCCAAGACATCCGCTGTTGAAAGTTT2840.1274983389300914No Hit
GCTGCGTTCTTCATCGATATGGGAGCCAAGACATCCACTGTTGAAAACTT2820.12660046330382316No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGCGATCCGTTGTTGATAGTTA2820.12660046330382316No Hit
GCTGCGTTCTTCATCGATATGGGAGCCAAGACATCCGCTGTTGAAAATTT2770.12435577423815253No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCAAGAGATCCATTGCTAAAAGTTG2670.11986639610681128No Hit
GCTGCGTTCTTCATCGATATGGGAGCCAAGCCATCCACTGTTGAAAATTT2580.11582595578860416No Hit
GCTGCGTTCTTCATCGATGCTATTGCAGTCTGTGTCCATTAGACACCTCC2380.10684719952592167No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAAGAAACTACTGGCAAACCG2320.10415357264711692No Hit
GCTGCGTTCTTCATCGGTATGGGAGCCAAGACATCCACTGTTGAAAATTT2230.1001131323289098No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)